4/16/2024 0 Comments Trump commander in chiefInstead of telling Trump what he wanted to hear, Selva was forthright. “So, what do you think of the parade?” Trump asked Selva. Trump did not understand that Kelly was being sarcastic. “Well, you know, General Selva is going to be in charge of organizing the Fourth of July parade,” he told the President. Kelly joked in his deadpan way about the parade. The subject came up again during an Oval Office briefing that included Trump, Kelly, and Paul Selva, an Air Force general and the vice-chairman of the Joint Chiefs of Staff. “In our society, there’s only one group of people who are more heroic than they are-and they are buried over in Arlington.” Kelly did not mention that his own son Robert, a lieutenant killed in action in Afghanistan, was among the dead interred there. Kelly could not believe what he was hearing. “This doesn’t look good for me.” He explained with distaste that at the Bastille Day parade there had been several formations of injured veterans, including wheelchair-bound soldiers who had lost limbs in battle. “Look, I don’t want any wounded guys in the parade,” Trump said. That was never clearer than when Trump told his new chief of staff, John Kelly-like Mattis, a retired Marine Corps general-about his vision for Independence Day. The divide was also a matter of values, of how they viewed the United States itself. Struggling to dissuade Trump, officials pointed out that the parade would cost millions of dollars and tear up the streets of the capital.īut the gulf between Trump and the generals was not really about money or practicalities, just as their endless policy battles were not only about clashing views on whether to withdraw from Afghanistan or how to combat the nuclear threat posed by North Korea and Iran. “I’d rather swallow acid,” his Defense Secretary, James Mattis, said. The generals, to his bewilderment, reacted with disgust. Sure enough, Trump returned to Washington determined to have his generals throw him the biggest, grandest military parade ever for the Fourth of July. The French general in charge of the parade turned to one of his American counterparts and said, “You are going to be doing this next year.” The event seemed to be calculated to appeal to Trump-his sense of showmanship and grandiosity-and he was visibly delighted. Vintage tanks rolled down the Champs-Élysées as fighter jets roared overhead. Macron staged a spectacular martial display to commemorate the hundredth anniversary of the American entrance into the First World War. In the summer of 2017, after just half a year in the White House, Donald Trump flew to Paris for Bastille Day celebrations thrown by Emmanuel Macron, the new French President.
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4/16/2024 0 Comments Sims 4 pose by pack modThis is where your own creativity and sorting conventions come into play. I also opt to put that in Brackets because…idk. I also further sort them by how many people are in the poses (couple, single, or group). I like to sort my poses by whether they’re story poses or model-type poses. ‘Amazing’ and ‘Barely amazing’ first, then and after it, while still keeping the titles with symbols in it in alphabetical order. So, for example, if you have poses labeled as followed: Pose Player sorts things alphabetically in game, prioritizing poses with with letters in the title first, then poses with symbols. This is where the ‘organization’ part comes in. without opening sims4studio) doesn’t do anything other than change the way it appears in the Mods folder. Renaming the file in the mods folder itself (a.ka. See that section that says ‘name’? That is the name of the package as it appears IN GAME and that is what you want to rename. this is basically a package editing tutorial.) and it will pop open in Sims 4 Studio. If you did, then you can double click a pose in your folder (or any package file for that matter. Make sure you’ve downloaded and installed Sims 4 Studio. DOUBLE CLICK A POSE TO OPEN IT IN SIMS4STUDIO For organization’s sake, I stick my poses into their own folder just so I can find them easier 2. You can make sub folders in your Mods folder. Usually you’ll find it in your main drive folder (C Drive, D Drive, whatever) unless you intentionally put it elsewhere. Navigate to your Electronic Arts > Sims 4 > Mods folder and drop all your poses in there. If this is your first time dealing with mods and poses, here’s how it goes: Sims 4 has a Mods folder that all that good stuff goes into. So *rosanna pansino voice* let’s get started! 1. (Tagging: to help spread this around!)Īnd that’s it. 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Species source of the secondary antibody: there is usually no predictable connection between species source and the quality of the secondary antibody. If the type of the primary antibody is not clear, IgG against the corresponding species can be used. If the primary monoclonal antibody is of a certain subclass of mouse IgG (IgG1, IgG2a, IgG2b, or IgG3), then almost all anti-mouse IgG can bind to it, or the secondary antibody can be selected to specifically target this subclass. If the primary antibody is mouse IgM, then the corresponding secondary antibody should be anti-mouse IgM. Polyclonal antibodies are mainly IgG immunoglobulins, so the corresponding secondary antibodies are anti-IgG antibodies. This is usually applicable for monoclonal antibodies. Type of primary antibody: the secondary antibody must match the class or subclass of the primary antibody. For example, if the primary antibody is a mouse-derived monoclonal antibody, an anti-mouse secondary antibody (goat anti-mouse or rabbit anti-mouse) should be selected. Species source of the primary antibody: the reactivity of the secondary antibody should be consistent with the species source of the primary antibody used. When selecting a secondary antibody, both the type of primary antibody and the requirements of subsequent detection schemes should be considered comprehensively: Usually, there are multiple secondary antibodies suitable for the subsequent detection of the target protein, and the selection can be optimized in specific experiments. If the results are highly correlated, then the antibody is validated for WB analysis. These methods include detection of whether the signal is eliminated or significantly reduced after genetic knockout or knockdown of the target gene analysis of the correlation between WB signals and signals of other detection methods (e.g., MS) in a set of different samples with variable expression of the target protein analysis of the correlation of protein levels by using two or more independent antibodies targeting different epitopes of the same protein expression of the target protein with a tag, and analysis of the correlation between antibody labeling and the detection of the tag. For unvalidated antibodies, there are suggested methodologies to validate the selectivity of the antibodies. There are currently databases that can be used for choosing characterized antibodies with high selectivities, such as Antibodypedia ( ), the Human Protein Atlas ( ), and the Antibody Registry ( ). The selectivity of antibodies directly affects WB results, and poor selectivity may lead to the misinterpretation of the results. This guide, therefore, aims to provide an updated and more concise and useable reference for future experiments and paper writing. Here, we will focus on some essential caveats during the WB experiment. However, concerns about WB have been voiced by many scientific journals in an effort to reduce potential mistakes and increase reproducibility. Therefore, WB remains the most commonly used methodology in the lab for protein detection. ELISA lacks loading controls, immunofluorescence is an in situ technique and is semiquantitative, while MS is expensive and depends on the experimental technique and conditions. Although there are many new alternative technologies, such as enzyme-linked immunosorbent assay (ELISA), immunofluorescence, and mass spectrometry (MS), they all have their own limitations to some extent. 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This paper describes a process to define a comprehensive list of exemplars for seven core Diagnostic and Statistical Manual (DSM) diagnostic criteria for autism spectrum disorder (ASD), and report on interrater reliability in applying these exemplars to determine ASD case classification. |
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